This one-day classroom-based course will introduce delegates to Flow Cytometry. We will cover the reagents used, how a cytometer works, experimental design, sample preparation and running, and data analysis and presentation. The course is aimed at those new to the field or those who want to increase their confidence in this technology. By the end of the course, delegates will be more confident in designing and running their own experiments.
9th March
5th October
As cytometers become capable of measuring an increasing number of fluorochromes, panel design becomes more complicated. It requires knowledge of the fluorochrome, the biology (antigen density, co-expression) and the sample preparation. In this one-day course we will look at the factors that need to be taken into account to build multicolour panels. We will do several interactive panel designs where we will use this knowledge to design panels of 6-10 and then 15+ colours. Course will include more advanced topics such as data spread, optical layout of multi-laser cytometers and presenting of data for publication.
The in person course includes refreshments, including lunch, morning and afternoon tea and coffee.
10th March
6th October
This one-day course will combine theory and practical. Delegates will learn what full spectrum cytometry can bring to their experiments. We will look at how full spectrum cytometers differ from conventional cytometers in their light detection, how this influences experimental design and some of the specific considerations of using this approach. We will run a simple experiment on spectral cytometers - the Cytek Aurora and the Sony ID7000.
8th October
Data analysis of flow data files can be daunting. Where do I set regions and gates? Which metric do I use?
In this one-day course we will guide delegates through the minefield of data analysis from simple gating to high dimensional analysis. This one-day course will introduce delegates to the analysis of flow data. We will cover the plot types, setting compensation, regions and gates, the metrics that can be derived, and data presentation. We will illustrate all these with data files that will be available to delegates.
13th March
7th October
Populations detected by analytical cytometry may be retrieved using droplet deflection cell sorting. Successful sorting requires knowledge of the sorting process and how to adjust parameters to maximise purity or yield There may be special considerations of downstream applications eg single cell sorting for RNAseq experiment. This two-day course will introduce delegates to the theory of cell sorting but will also include a practical element where we will use a cuvette-based system (FACSAria Fusion), a stream-in-air system (Thermo Bigfoot) and an imaging sorter (FACSDiscover S8).
11th & 12th March
This Practical Imaging Flow Cytometry Course is new course for Autumn of 2025. The course can be taken as a standalone one day course or can be taken as a building block to add to your knowledge from our other flow cytometry courses. The course starts with an introduction to Imaging Cytometry, including information about many of the commercially avalaible image cytometers. There will be practicals in Babraham Â鶹ÊÓƵ Flow Cytometry Facility on the Thermo Attune CytPix, Cytek Amnis Imagestream MkII and BD FACSDiscover S8 spectral cell sorter with imaging capabilities. The day will will conclude with sessions on data analysis from these systems and an overview of presentation of imaging data.
9th October
Discounts given, use the appropriate code on Eventbrite:
10% discount is given to delegates who work in companies on the Babraham Research Campus (use code BRC10)
50% discount given to academics (use code academic50)
20% discount given to companies booking multiple people onto the same course or the same person on multiple courses (use code Multi20 ).
The March and October courses can be taken as individual courses or in a block.
After 10 years and following the training of over 2000 individual scientists from not just the UK but worldwide, the highly successful Babraham Â鶹ÊÓƵ Flow Cytometry Courses have been renewed and updated to include the most up to date information about this fast moving technology. These new courses are now part of the 'BuildingCyt' programme which aims to build cytometry knowledge.
Flow Cytometry has been established in research and clinical laboratories for over 40 years and is a crucial technique in many investigations. However, the appreciation of how cytometers work and how best to set up and optimise an experiment is often lacking. Established in 2014, these highly successful courses are designed and run by experts in flow cytometry and are aimed at those relatively new to the field.
The modular design of the courses allows delegates to build on knowledge from one course to the next, giving them the opportunity to implement ‘best practices’ and ultimately improving their flow cytometry experiments. It is recommended that those new to the field attend the Foundation course before the follow-up courses.
The flow cytometry courses at BI are interactive, lecture-, practical- and exercise-based courses given by experts in the field who can share their in-depth knowledge of an evolving technology. These courses are ideal for those using Flow Cytometry in Industry and Academia. These courses have been designed to build up knowledge of flow cytometry to ensure that the delegate is confident to design, carry out, analyse and present their flow cytometry data. Utilising the state-of-the-art instrumentation within the Babraham Â鶹ÊÓƵ Flow Cytometry Facility many of these courses have a practical element using cytometers within the facility.
'Instructors were knowledgeable and engaging. Course material was well thought out and well presented' Introductory course October 2025
'Very good pace and structure. The mini quizzes helped consolidate our knowledge and the exercises in particular were very useful for consolidation' Introductory course October 2025
'Having done some flow before, this course was great at refreshing my existing knowledge and gave me some new knowledge around analysis and interpreting data' Introductory course October 2025
'A fantastic course for a deeper dive into flow and best practices!' Advanced course October 2025
'This was the ideal course to get started with spectral flow cytometry. Given by people that are really experts in this field.' Spectral Flow Cytometry Course October 2025
'The cell sorting course was an excellent run through the theory & practical aspects of sorting on various cell sorters. The teaching was excellent and they were able to answer all my burning questions' Cell Sorting Course January 2025
'A thoroughly comprehensive course on data analysis. The instructors were fantastic and could tailor examples to your areas of interest.' Data analysis course October 2025