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BuildingCyt Flow Cytometry Courses

Training course 2018

2024 Flow Cytometry Courses

 


Foundation Block: Introduction to Flow (24-27 delegates)

This one-day classroom-based course will introduce delegates to Flow Cytometry. We will cover the reagents used, how a cytometer works, experimental design, sample preparation and running, and data analysis and presentation. The course is aimed at those new to the field or those who want to increase their confidence in this technology. By the end of the course, delegates will be more confident in designing and running their own experiments.

  • Fluorescence and fluorochromes
  • How a Flow Cytometer works
  • Compensation
  • Experimental design
  • Sample preparation
  • Data analysis
  • Data presentation

25th January 2024

18th April 2024

1st October 2024

13th November 2024

 


Advanced Flow Cytometry : Multicolour and Panel design (15 delegates)

As cytometers become capable of measuring an increasing number of fluorochromes, panel design becomes more complicated. It requires knowledge of the fluorochrome, the biology (antigen density, co-expression) and the sample preparation. In this one-day course we will look at the factors that need to be taken into account to build multicolour panels. We will do several interactive panel designs where we will use this knowledge to design panels of 6-10 and then 15+ colours. Course will include more advanced topics such as data spread, optical layout of multi-laser cytometers and presenting of data for publication.

The in person course includes refreshments, including lunch, morning and afternoon tea and coffee.

  • Fluorochromes
  • Compensation and data spread
  • Cytometer layout
  • Antigen density
  • Staining pattern
  • Fixation/Sample prep
  • Design considerations
  • Publication of flow data

7th March 2024

28th May 2024

2nd October 2024

3rd December 2024

 


Building Block: Spectral analysis by Flow Cytometry (15 delegates)

This one-day course will combine theory and practical. Delegates will learn what full spectrum cytometry can bring to their experiments. We will look at how full spectrum cytometers differ from conventional cytometers in their light detection, how this influences experimental design and some of the specific considerations of using this approach. We will run a simple experiment on two spectral cytometers - the Cytek Aurora and the Sony ID7000.

  • Fluorescence
  • Filters/lasers/detectors
  • Unmixing
  • Exp design

14th February 2024

25th June 2024

4th October 2024

 


Building Block: Flow Cytometric Data analysis (10 delegates)

Data analysis of flow data files can be daunting. Where do I set regions and gates? Which metric do I use?

In this one-day course we will guide delegates through the minefield of data analysis from simple gating to high dimensional analysis. This one-day course will introduce delegates to the analysis of flow data. In the first section, we will cover the plot types, setting compensation, regions and gates, the metrics that can be derived, and data presentation. The second section will look at the types of high dimensional analysis that are available, specifically dimensionality reduction and clustering methods. We will illustrate all these with data files that will be available to delegates.

  • Which plots to use
  • Examples of gating
  • Metrics
  • Dim reduction and clustering
  • Hands on analysis using FlowJo and R

28th March 2024

3rd October 2024

 


Building Block : Practical Cell sorting  (12 delegates)

Populations detected by analytical cytometry may be retrieved using droplet deflection cell sorting. Successful sorting requires knowledge of the sorting process and how to adjust parameters to maximise purity or yield There may be special considerations of downstream applications eg single cell sorting for RNAseq experiment. This two-day course will introduce delegates to the theory of cell sorting but will also include a practical element where we will use a cuvette-based system (FACSAria Fusion), a stream-in-air system (Thermo Bigfoot) and an automated system (Sony SH800).

  • Day One am: Refresher of how a cytometer works; nozzles; cuvette v stream in air; drop formation; drop delay
  • Day One pm: Practical – rotate between sorters– System walkthrough; setting drop delay/sorter set up
  • Day Two am: Sort masks; sample prep; other considerations
  • Day Two pm: Sorting on different instruments

10th & 11th January 2024

12th & 13th March 2024

 


A 10% discount is given to delegates who work in companies on the Babraham Research Campus and a 50% discount given to academics.

After 9 years and following the training of over 2000 individual scientists from not just the UK but worldwide, the highly successful Babraham 鶹Ƶ Flow Cytometry Courses have been renewed and updated to include the most up to date information about this fast moving technology.  These new courses are now part of the 'BuildingCyt' programme which aims to build cytometry knowledge.

Flow Cytometry has been established in research and clinical laboratories for over 40 years and is a crucial technique in many investigations. However, the appreciation of how cytometers work and how best to set up and optimise an experiment is often lacking. Established in 2014, these highly successful courses are designed and run by experts in flow cytometry and are aimed at those relatively new to the field.

The modular design of the courses allows delegates to build on knowledge from one course to the next, giving them the opportunity to implement ‘best practices’ and ultimately improving their flow cytometry experiments. It is recommended that those new to the field attend the Foundation course before the follow-up courses.

The flow cytometry courses at BI are interactive, lecture-, practical- and exercise-based courses given by experts in the field who can share their in-depth knowledge of an evolving technology.   These courses are ideal for those using Flow Cytometry in Industry and Academia. These courses have been designed to build up knowledge of flow cytometry to ensure that the delegate is confident to design, carry out, analyse and present their flow cytometry data.  Utilising the state-of-the-art instrumentation within 鶹Ƶ Flow Cytometry Facility many of these courses have a practical element using cytometers within the facility.

 


Testimonials from past courses:

'Great course leaders. Great course contents, great instructors Rachael and Derek delivered the course at the right speed, the workbook and exercise on panel design was very helpful to aid learning, thank you very much! Looking forward to the next course.'

'Great course that covers the basics and principles of flow cytometry for non-expert users.'

'Clickers kept the course engaging. Leaders are certainly experts in their field. The learning involved in this course is logical, engaging and extremely clear!'

'Ideal to go over important theoretical topics. The positive vibe is really nice to keep us focussed’

 


Terms and Conditions of Flow Cytometry Courses run by Babraham 鶹Ƶ Enterprise Ltd (BIE)

  • No refunds, transfers or deferrals can be made if less than 14 days’ notice is given.
  • BIE reserves the right to cancel, reschedule or change the location or content of a course without penalty if considered necessary.
  • BIE undertakes to inform customers as soon as the change is made and customers may book an alternative course at no extra charge, or receive a full refund or credit against future courses.
  • If a customer wishes to cancel a course, provided more than 14 days’ notice prior to the course start date is given, the tuition fee will be refunded less a £50 cancellation fee.
  • If a customer wishes to transfer to another course, provided more than 14 days’ notice prior to the course start date is given and subject to availability, no additional charge will be made, other than for any new study materials that may be required.
  • It may be possible to transfer your place on a course to another member of your organisation, but this will be assessed by BIE on a case to case basis.